Study Detail

TitleCo-evolution of Transcriptional Silencing Proteins and the DNA Elements Specifying Their Assembly
Study TypeEpigenetics
Abstract Co-evolution of transcriptional regulatory proteins and their sites of action has been often hypothesized but rarely demonstrated. Here we provide experimental evidence of such co-evolution in yeast silent chromatin, a finding that emerged from studies of hybrids formed between two closely related .. [more]
Description Chromatin immunoprecipitation of either Sc-Sir4 or Sb-Sir4 was performed in five separate S. cerevisiae/S. bayanus hybrid diploid strains. Each Sir4 allele was separately myc-tagged, and the ChIPs were performed using anti-c-myc affinity resin. Input and IP fractions for each ChIP experiment were .. [more]
Center NameUC-Berkeley