Sample Detail

TitleE-MTAB-1674:pMad 2 to 2.5 hr IP exp1
DescriptionProtocols: Embryos were collected from yw adults as in Saunders et al. 2013. Saunders et al. Extensive polymerase pausing during Drosophila axes patterning enables high-level and pliable transcription. 2013. Genes and Development. Published online May 15, 2013. 215459.113. Embryo ChIP was performed as described in Sandmann et al. 2006, except that 5% formaldehyde was used in the crosslinking solution. For PI samples, the guinea pig pre-immune serum was used instead of pMad antibody. Samples were sonicated for 30 min using a Bioruptor with 9 sec on, 1 min off cycles, on high-energy settings. Two independent biological replicates were performed for each ChIP at each time point. Sandmann T, Jakobsen JS, Furlong EE. ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos. 2006. Nature Protocols 1:2839-2855.

Organism Info

Taxon ID7227
Common Name
Scientific NameDrosophila melanogaster
Anonymized Name
Individual Name