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SRX1626306

FASTQ

SRA

Experiment Detail

TitleMethylC-seq of Gossypium barbadense
Design DescriptionTotal genomic DNA (~5 ?g) was fragmented to 100–1000 bp using Bioruptor (Diagenode, Denville, New Jersey). End repair (NEBNextŽ End Repair Module) was performed on the DNA fragment followed by adding an 'A' base to the 3' end (NEBNextŽ dA-Tailing Module), and the resulting DNA fragment was ligated to the methylated DNA adapter (NEXTflex™ DNA Barcodes, Bioo Scientific, Austin, Texas). The adapter-ligated DNA of 200–400 bp was purified using AMPure beads (Beckman Coulter, Brea, California), followed by sodium bisulfite conversion using MethylCode™ Bisulfite Conversion Kit (Life Technologies, Foster City, California). The bisulfite-converted DNA was amplified by 12 cycles of PCR using LongAmpŽ Taq DNA Polymerase (NEB, Ipswich, Massachusetts) and purified using AMPure beads (Beckman Coulter, Brea, California). The Paired-End sequencing of the MethylC-seq libraries was performed for 126 cycles.
OrganismGossypium barbadense

Library Description

Namecultivated Gossypium barbadense_Rep2
StrategyBisulfite-Seq
SourceGENOMIC
SelectionRANDOM PCR
LayoutPAIRED
Orientation
Nominal Length
Nominal Sdev
Construction Protocol

Platform

PlatformILLUMINA
Instrument ModelIllumina HiSeq 2500

Processing

Base Calls
Sequence Space
Base Caller

Spot Information

Number of Reads per Spots0
Spot Length0

Read Spec

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Submission SRA384261 FTP
Study SRP071640
Sample SRS1335799
Run SRR3219095 FASTQ SRA
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