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DRX012752

FASTQ

SRA

Experiment Detail

Title1C+aAM
Design Descriptionalpha-Amanitin treated 1-cell (1C) embryos
OrganismMus musculus

Library Description

Name
StrategyRNA-Seq
SourceTRANSCRIPTOMIC
SelectioncDNA
LayoutPAIRED
Orientation
Nominal Length200
Nominal Sdev50.0
Construction ProtocolOne-cell embryos were transferred to 20 ul drop of M16 medium containing 10 ug/ml alpha-amanitin (Sigma A2263) three hours after insemination. Total RNA was prepared from about 2300 first mitosis-arrested embryos using ISOGEN II (NIPPON GENE). For RNA-seq library construction, we performed TruSeq Standard Total RNA Sample Prep Kit (Illumine, San Diego, CA #RS-122-2201 and 2202) and prepared 600 ng RNAs.

Platform

PlatformILLUMINA
Instrument ModelIllumina HiSeq 2500

Processing

PipeSection
Step Index1
Prev Step IndexNIL
Program
Version

Spot Information

Number of Reads per Spots0
Spot Length202

Read Spec

Read Index 0
Read Label
Read ClassApplication Read
Read TypeForward
Base Coord1
Read Index 1
Read Label
Read ClassApplication Read
Read TypeReverse
Base Coord102

Navigation

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Study DRP001110
Sample DRS012552
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