FASTQExperiment Detail
| Title | 1C+aAM |
| Design Description | alpha-Amanitin treated 1-cell (1C) embryos |
| Organism | Mus musculus |
Library Description
| Name | |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | cDNA |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 200 |
| Nominal Sdev | 50.0 |
| Construction Protocol | One-cell embryos were transferred to 20 ul drop of M16 medium containing 10 ug/ml alpha-amanitin (Sigma A2263) three hours after insemination. Total RNA was prepared from about 2300 first mitosis-arrested embryos using ISOGEN II (NIPPON GENE). For RNA-seq library construction, we performed TruSeq Standard Total RNA Sample Prep Kit (Illumine, San Diego, CA #RS-122-2201 and 2202) and prepared 600 ng RNAs. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2500 |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 202 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 102 |
