Title | 1C+aAM |
Design Description | alpha-Amanitin treated 1-cell (1C) embryos |
Organism | Mus musculus |
Name | |
Strategy | RNA-Seq |
Source | TRANSCRIPTOMIC |
Selection | cDNA |
Layout | PAIRED |
Orientation | |
Nominal Length | 200 |
Nominal Sdev | 50.0 |
Construction Protocol | One-cell embryos were transferred to 20 ul drop of M16 medium containing 10 ug/ml alpha-amanitin (Sigma A2263) three hours after insemination. Total RNA was prepared from about 2300 first mitosis-arrested embryos using ISOGEN II (NIPPON GENE). For RNA-seq library construction, we performed TruSeq Standard Total RNA Sample Prep Kit (Illumine, San Diego, CA #RS-122-2201 and 2202) and prepared 600 ng RNAs. |
Platform | ILLUMINA |
Instrument Model | Illumina HiSeq 2500 |
PipeSection | |
---|---|
Step Index | 1 |
Prev Step Index | NIL |
Program | |
Version |
Number of Reads per Spots | 0 |
Spot Length | 202 |
Read Index 0 | |
---|---|
Read Label | |
Read Class | Application Read |
Read Type | Forward |
Base Coord | 1 |
Read Index 1 | |
Read Label | |
Read Class | Application Read |
Read Type | Reverse |
Base Coord | 102 |