BioSample Detail : SAMD00000688

Description

TitleK4B-ES_Dplus_H3K9me3
Taxonomy ID39442
Organism NameMus musculus musculus

General Info

OrganizationDepartment of Reproductive Biology

Model

Sample typeGeneric

Attributes

sample_nameDRS011760
sample commentES cells carrying a doxycycline-inducible Kdm4b transgene was created using a piggyBac transposon-based expression system from the NCH.4.6 male mouse ES cell line (C57B6/N x C57B6/N) with normal karyotype, and named K4B-ES cells.K4B-ES cells were cultured in the presence or absence of doxycycline, and subjected to fixed chromatin preparation. The fixed chromatin samples (Dminus and Dplus) were used for chromatin immuno-precipitation using anti-histone H3K9me3 polyclonal antibody (Abcam ChIP-grade ab8898). ChIP-Seq libraries were prepared for the immuno-precipitated DNA as well as the input DNA of Dminus and D plus samples using the NEBNext® ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (NEB) according to manufacturer's instructions.