BioSample Detail : SAMD00000639


TitleNormalized cDNA library sequencing using 454 pyrosequencing technology in Hodotermopsis sjostedti
Taxonomy ID377914
Organism NameHodotermopsis sjostedti

General Info

OrganizationLaboratory of Ecological Genetics, Graduate School of Environmental Science, Hokkaido University


Sample typeGeneric


sample commentHodotermopsis sjostedti collected from Yaku-shima Island were used. Total RNAs from various castes/developmental stages were mixed. For construction of cDNA library for 454 pyrosequencing, the mixed total RNA was subjected to first-strand cDNA synthesis with a oligo-dT primer, cap-trapping, second strand cDNA syntheis, and normalization.