Recently, high-throughput sequencing technology is rapidly improving in quality, speed and cost. It is, therefore, becoming more widely used to study on whole bacterial communities.
Rhizobial inoculation was often unsuccessful in legume fields, because soil native strains highly competed with the introduced inoculants. Conventional methods to monitor the behavior of inoculants are likely impractical and time-consuming in agricultural fields. Thus, We analyzed metagenome of the bacterial community associated with root nodules of soybean (Glycine max [L.] Merr., var. Tachinagaha) in order to descriminate the inoculant population from soil native population of soybean bradyrhizobia in an agricultural soil by mapping metagenome reads to the representative soybean bradyrhizobial genomes.