SRP268069

Title	De novo reconstruction of transcriptomes of ten Oryza sativa cultivars using PacBio Single-Molecule Real-Time sequencing
Study Type	Other
Abstract	We sequenced transcriptomes of ten rice cultivars of the subspecies aus, indica and japonica on the PacBio Sequel I platform. RNA was isolated from different tissues of plants grown under control and abiotic stress conditions in climate chambers and in the field. De novo reference transcriptomes wer .. [more]e reconstructed resulting in 38,900 to 54,500 high-quality isoforms per cultivar.The newly generated rice transcriptomes are useful to identify candidate genes for stress tolerance breeding that are not present in the reference transcriptome/genome. In addition, our approach provides a general, cost-effective alternative to genome sequencing for the identification of candidate genes in highly stress tolerant "exotic" genotypes. [less]
Description
Center Name	BioProject

DRASearch