| Abstract |
RNAseq was performed on several barley accessions to understand their diversity, with an emphasis on the class of plant immune receptors that contain nucleotide-binding, leucine-rich repeats (NB-LRR). The accessions were sequenced at either 100 bp or 150 bp paired end, except CI 16147 which had 150 .. [more]bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.32) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters. [less]
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FTP
