Study Detail

TitleMaize Mu transposon flanking sequences
Study TypeOther
Abstract The Mu transposon system of maize is highly active, with each of the ~50-100 copies transposing on average once each generation. The approximately one dozen distinct Mu transposons contain highly similar ~215 bp Terminal Inverted Repeats (TIRs) and generate 9-bp Target Site Duplications (TSDs) upon .. [more]
Description DLA, a genome walking method, was adapted for sequencing Mu flanking sequences using the 454 FLX platform. Mu flanking sequences from different Mu lines were amplified by a genome walking method, DLA (Liu et al., Genetics, Submitted) and pooled for the 454 pyrosequencing. The primers used to constr .. [more]
Center NameIowa State University