| Title | Genome-wide DNA methylation profile in mutants of a rice HISTONE DEACETYLASE (HDA705) |
| Study Type | Other |
| Abstract |
Plants perceive and respond to environmental stresses, and reversible and plastic characteristics of the responses are key mechanisms for surviving under ever-changing environmental conditions. Histone acetylation has been known as a quick and reversible switch for regulation of gene expression netw .. [more]ork and, in plants, it is one of the major components in environmental stress responses. In addition, histone modification is tightly connected to DNA methylation in the genome, and therefore it could contribute to long-term memory of environmental responses through stable inheritance of altered DNA methylation states in stress responsive genes. In this study, we isolated rice mutants of a HISTONE DEACETYLASE gene (HDA705) that is the closest HDA to HDA6 in Arabidopsis thaliana, and characterized changes in DNA methylation in the genome by comparing with those of met1 and ddm1 mutants. In contrast to met1 and ddm1, genome-wide reduction in CG and/or CHG methylation was not evident in hda705. Instead, hda705 induces a genome-wide increase of CHH methylation that are often associated with similar increases in CHH methylation in met and/or ddm1. CHH methylation in rice might compensate the deficiency in CG and CHG methylation in met1 and ddm1 in a subset of regions in the genome, and deficiency of histone deacetylation by HDA705 might give a similar effect on CHH methylation as those in met1 and ddm1 without apparent loss of CG and/or CHG methylation. [less]
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| Description |
Plants perceive and respond to environmental stresses, and reversible and plastic characteristics of the responses are key mechanisms for surviving under ever-changing environmental conditions. Histone acetylation has been known as a quick and reversible switch for regulation of gene expression netw .. [more]ork and, in plants, it is one of the major components in environmental stress responses. In addition, histone modification is tightly connected to DNA methylation in the genome, and therefore it could contribute to long-term memory of environmental responses through stable inheritance of altered DNA methylation states in stress responsive genes. In this study, we isolated rice mutants of a HISTONE DEACETYLASE gene (HDA705) that is the closest HDA to HDA6 in Arabidopsis thaliana, and characterized changes in DNA methylation in the genome by comparing with those of met1 and ddm1 mutants. In contrast to met1 and ddm1, genome-wide reduction in CG and/or CHG methylation was not evident in hda705. Instead, hda705 induces a genome-wide increase of CHH methylation that are often associated with similar increases in CHH methylation in met and/or ddm1. CHH methylation in rice might compensate the deficiency in CG and CHG methylation in met1 and ddm1 in a subset of regions in the genome, and deficiency of histone deacetylation by HDA705 might give a similar effect on CHH methylation as those in met1 and ddm1 without apparent loss of CG and/or CHG methylation. [less]
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| Center Name | NARO |
FTP
