Study Detail

TitleInferring the choreography of parental genomes during fertilization with ultra-large-scale whole-transcriptome analysis
Study TypeTranscriptome Sequencing
Abstract Fertilization precisely choreographs parental genomes by utilizing gamete-derived cellular factors and by activating genome regulatory programs. Here, we carefully extracted more than 14 x10,000 high-quality mouse metaphase II oocytes with which we established the transcriptional profile of four ear .. [more]
Description We collected MII oocytes from the oviducts of 8- to 12-week-old superovulated female BBF1 mice, which are F1 progeny from a cross between Balb/c females and C57BL/6 males. After removing cumulus cells, we manually picked high-quality cells (~30 oocytes per mouse). The 1C, 2C, and 4C embryos were obt .. [more]
Center NameUT_HGC