| Abstract |
A balanced fertilization can increase crop yields partly due to stimulating microbial activities and growths. In this study, we tried to investigate arbuscular mycorrhizal fungi (AMF) in arable soils to guide for a more effective fertilization. We applied pyrosequencing-based approach to study AMF d .. [more]iversity, as well as their responses to different long-term (over 20 years) fertilizations, including organic manure (OM) and mix chemical fertilizers of NP, NK and NPK. Data analysis revealed that 124998 of 18S rRNA gene fragments were dominated by Glomeromycota with 59611 sequences, generating 70 operational taxonomic units (OTUs), of which the three largest families were Glomeraceae, Gigasporaceae and Acaulosporaceae. Compared by P-deficiency fertilizations, Control and NK, AMF diversity and richness significantly decreased under long-term P fertilizations, such as NP, NPK and OM. Concomitantly, the AMF community structure shifted. Supported by canonical correspondence analysis, we hereby propose that long-term balanced fertilization, especially P fertilizer, helps the build-up of soil nutrients. Consequently, some AMF community constituents are sacrificed, propelled by the self-regulation of plant-AMF-microbes system, resulting in an agro-ecosystem with a better sustainability. This knowledge would be valuable towards better understandings of not only AMF community in agro-ecosystem, but also the balanced fertilization impacting agro-ecosystem. [less]
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