Study Detail

TitleUnamplified Cap Analysis of Gene Expression on a single molecule sequencer (HeliScopeCAGE)
Study TypeTranscriptome Analysis
Abstract We report the development of a simplified Cap Analysis of Gene Expression (CAGE) protocol adapted for single molecule sequencers which avoids second strand synthesis, ligation, digestion and PCR. HeliScopeCAGE directly sequences the 3’ end of cap trapped first strand cDNAs. As with previous versio .. [more]
Description HeliScopeCAGE protocol: First strand cDNA is generated from total RNA using an excess of random primer. 5’ end complete first strand cDNAs are captured for capped RNAs. First strand cDNA is polyA tailed and blocked and then loaded directly onto the HeliScope flow cell for sequencing. Data proces .. [more]
Center NameRIKEN_OSC