Study Detail

TitleLinking new promoters to functional transcripts in small samples with nanoCAGE and CAGEscan
Study TypeTranscriptome Analysis
Abstract Sequencing-based analysis of 5′ capped ends of RNAs have unveiled an unexpected complexity of the mammalian transcriptome, in which coding and non-coding RNAs produced in loci often harboring multiple promoters coexist with processed RNAs that exhibit a variety of 5′ modifications. In light of .. [more]
Description While the classic CAGE protocol requires a substantial number of biochemical processing steps, CAGEscan and nanoCAGE take advantage of a peculiar property of reverse transcriptase, called “template switching” to select 5′ ends of capped transcripts. This property allows proceeding directly fr .. [more]
Center NameRIKEN_OSC