DRP000087

Title	Survey of short RNA expression after interference of RNAs expressed from retrotransposons
Study Type	Transcriptome Analysis
Abstract	To assess the regulatory role of LINE-1 teRNAs in myogenesis - and its disruption by LINE-1 RNAi - we generated genome-wide maps of transcriptional activity in C2C12 muscle cells (myoblasts) poised for differentiation. 18 million Cap Analysis Gene Expression (CAGE .. [more]) tags were sequenced using an updated protocol that incorporated massively parallel sequencing and tags extended to an average length of 27nt. [less]
Description	Here, we report in detail the first use of RNA interference against LINE-1 transposed element RNAs, in an effort to ascertain their functional significance. Using mouse myoblasts as a model system, we observed clear phenotypic consequences post knock-down of LINE- .. [more]1 transcription. Deep sequencing of capped mRNAs and short RNAs was then used to survey transcriptional activity in wild-type and LINE-1 knock-down myoblasts, revealing strong expression changes within the gene networks that govern muscle cell differentiation, thousands of differentially expressed LINE-1 copies and evidence for a global mechanism by which LINE-1 RNAs may regulate protein-coding gene expression. [less]
Center Name	RIKEN_OSC

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