| Description |
Although recent studies have revealed that the majority of mouse genes
are subjected to regulation of alternative promoters (APs), the biological relevance of
this phenomenon remains unclear. To enable more comprehensive TSS analysis in the
respective cell types, .. [more]we recently developed a method,combining oligo-capping with the
massively paralleled sequencing technology,Illumina GA. In this method, which we named
TSS Seq, sequence adaptor which is necessary for Illumina GA sequencing is directly
introduced to the cap site of the mRNA. By sequencing 36-48 sequence immediately
downstream of the TSSs (TSS tags), it is possible to obtain precise positional
information of transcriptional start sites (TSSs). [less]
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FTP
