| Title | Comprehensive analysis of polysomal mRNAs in HT29 cell at 4hr after treatment with tunicamycin. |
| Study Type | Transcriptome Analysis |
| Abstract |
Expression profile in HT29 cell exposed to ER stress was attempted. We used shotgun sequencing method, in which next gene sequencing technology and polysome analysis were combined.
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| Description |
We analyzed the mRNA profile in cytoplasm and in polysome during the endoplasmic reticulum (ER) stress using second generation sequencers. We compared three time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ??g/mL ER stress inducible agent .. [more]Tunicamycin (Tm)). This is the first time mRNA in ???polysome??? is analyzed using the next generation sequencers and compared with that of cytoplasm. [less]
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| Center Name | UT-MGS |
FTP
