| Description | General description. Rice plants (Oryza sativa L. cultivar “Nipponbare”) were cultivated in paddy fields dressed with low N (LN; 0 kg ha-1). The roots (approximately 100 g) were sampled on August 26, 2009 and homogenized without surface sterilization to prepare the root-associated bacterial cells, and the bacterial cells were extracted and purified using a bacterial cell enrichment method. Total DNA was extracted from the cell fraction. We amplified 16S rRNA genes using a primer set 27F, 518R. These PCR primers target the V1 and V3 region.
Field metadata.
Country: Japan
Latitude: 38-27-39.37’N
Longitude: 141-5-33.33-E
Altitude: 4 m a.s.l.
Classification: Gray lowland soil (classification of cultivated soils in Japan, 3rd approximation)
Geomorphic Position: Alluvial plain
Slope Characteristics: Flat
Precipitation: Udic soil moisture regime
Water Table Depth: 97 cm
Drainage: Poorly drained
Land Use: Paddy rice field
Parent material: Unconsolidated alluvium
Vegetation: Paddy field after harvest |
FTP
