Dark nodules occurring in Hypericum perforatum at defined locations in several tissues are the sites of hypericin accumulation and most likely hypericin synthesis. To identify transcripts of genes potentially in involved in hypericin synthesis, cDNA libraries from petal rims with dark nodules and petal centers without dark nodules (field grown plants) have been sequenced using a paired-end approach (2x 101 nt) on a HiSeq2000 sequencer.