Experiment Detail
| Title | Axenic Chlorella variabilis F275 RNA-seq |
| Design Description | The algal cells of axenic C. variabilis F275 was collected on sterilized polycarbonate membrane filters (47 mm diameter, 0.45m, Jinlong company, China). Total RNA was isolated with an easy-spin IIp Plant RNA Extraction Kit (Biomiga, Inc) and the RNA concentration and quality was assessed using NanoDrop 2000 (Thermo), Qubit 2.0 Fluorometer (Life Technologies) and an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). The cDNA is then made using random hexamers or oligo(dT) primers and the sequencing was performed using Illumina HiSeqTM 2000 in Novogene Bioinformatics Technology Co. (Beijing, China). |
| Organism | Chlorella variabilis |
Library Description
| Name | Axenic Chlorella variabilis F275 |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | RANDOM |
| Layout | SINGLE |
| Construction Protocol |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | SRA912567 |
 FTP
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Study | SRP211746 | ||
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Sample | SRS5014810 | ||
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Run | SRR9526536 |
FASTQ
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SRA
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