Experiment Detail
| Title | Skud-RNA-rep1-pA |
| Design Description | Total RNA was prepared by bead-beating cells with acid phenol / chloroform and precipitation. PolyA RNA was selected using oligo-dT beads. RNA was fragmented and reverse-transcribed with a primer containing illumina library priming sites and ending with oligodTV. The resulting cDNA was circularized and PCR amplified to generate barcoded Illumina libraries. For more see Yoon et al., RNA 2010 and Spealman et al., Meth Mol. Biol 2016). |
| Organism | Saccharomyces kudriavzevii |
Library Description
| Name | Sk-pA-rep1 |
| Strategy | OTHER |
| Source | TRANSCRIPTOMIC |
| Selection | RACE |
| Layout | SINGLE |
| Construction Protocol |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2000 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | SRA573988 |
 FTP
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Study | SRP109132 | ||
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Sample | SRS2282578 | ||
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Run | SRR5681093 |
FASTQ
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SRA
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