Title | Spar-RNA-rep1-TSS |
Design Description | Total RNA was prepared by bead-beating cells with acid phenol / chloroform and precipitation. PolyA RNA was selected using oligo-dT beads. RNA was fragmented and a 5' phosphates were removed with CIP, then 5' caps removed with TAP. 5' and 3' adapters were ligated to remaining RNA and library was PCR-amplified. For more see Arribere et al., Genome Research 2013). |
Organism | Saccharomyces paradoxus |
Name | Sp-TSS-rep1 |
Strategy | OTHER |
Source | TRANSCRIPTOMIC |
Selection | CAGE |
Layout | SINGLE |
Construction Protocol |
Platform | ILLUMINA |
Instrument Model | Illumina HiSeq 2000 |
Base Calls | |
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Sequence Space | |
Base Caller |
Number of Reads per Spots | 0 |
Spot Length | 0 |