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SRX2916424

FASTQ

SRA

Experiment Detail

TitleSpar-RNA-rep1-TSS
Design DescriptionTotal RNA was prepared by bead-beating cells with acid phenol / chloroform and precipitation. PolyA RNA was selected using oligo-dT beads. RNA was fragmented and a 5' phosphates were removed with CIP, then 5' caps removed with TAP. 5' and 3' adapters were ligated to remaining RNA and library was PCR-amplified. For more see Arribere et al., Genome Research 2013).
OrganismSaccharomyces paradoxus

Library Description

NameSp-TSS-rep1
StrategyOTHER
SourceTRANSCRIPTOMIC
SelectionCAGE
LayoutSINGLE
Construction Protocol

Platform

PlatformILLUMINA
Instrument ModelIllumina HiSeq 2000

Processing

Base Calls
Sequence Space
Base Caller

Spot Information

Number of Reads per Spots0
Spot Length0

Read Spec

Navigation

Submission SRA573988 FTP
Study SRP109132
Sample SRS2282571
Run SRR5681106 FASTQ SRA
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