Experiment Detail
| Title | GSM2443081: MadC-mycelium-Dark-R2; Phycomyces blakesleeanus; RNA-Seq |
| Design Description | |
| Organism | Phycomyces blakesleeanus |
Library Description
| Name | |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | cDNA |
| Layout | SINGLE |
| Construction Protocol | Mycelia were disrupted by two 0.5-min pulses in a cell homogenizer (FastPrep-24, MP Biomedicals) with 1.5 g of zirconium beads (0.5 mm diameter) using the RNeasy Plant Mini Kit (Qiagen) following the manufacturer procedure. Libraries for RNAseq were prepared with RNA from mycelia of the wild type and the madC mutant strains using the TruSeq kit protocol (Illumina). |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina HiSeq 2500 |
Processing
| Base Calls | |
|---|---|
| Sequence Space | |
| Base Caller | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
Navigation
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Submission | SRA519090 |
 FTP
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Study | SRP095996 | ||
|
Sample | SRS1892622 | ||
|
Run | SRR5141331 |
FASTQ
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SRA
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