FASTQExperiment Detail
| Title | YamamotoYoshidayama__AGTCGAGA__ITS |
| Design Description | Each root sample was washed in 70% ethanol using TissueLyser II (QIAGEN) and 1-mm zirconium balls. The washed roots were cooled down at -20 degrees C and were pulverized using TissueLyser II and 4-mm zirconium balls. Metagenomic DNA was extracted from each root by CTAB method. |
| Organism | rhizosphere metagenome |
Library Description
| Name | YamamotoYoshidayama__AGTCGAGA__ITS |
| Strategy | AMPLICON |
| Source | METAGENOMIC |
| Selection | PCR |
| Layout | SINGLE |
| Construction Protocol |
Platform
| Platform | LS454 |
| Instrument Model | 454 GS Junior |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Technical Read |
| Read Type | Adapter |
| Base Coord | 0 |
| Read Index 1 | |
| Read Label | |
| Read Class | Technical Read |
| Read Type | BarCode |
| Base Coord | 0 |
| Read Index 2 | |
| Read Label | |
| Read Class | Technical Read |
| Read Type | Primer |
| Base Coord | 0 |
| Read Index 3 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 31 |
