FASTQExperiment Detail
| Title | PCR amplification of 16S rRNA gene (V1-V2 region) sequence |
| Design Description | |
| Organism | human gut metagenome |
Library Description
| Name | |
| Strategy | AMPLICON |
| Source | METAGENOMIC |
| Selection | PCR |
| Layout | SINGLE |
| Construction Protocol | PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5?-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3?) containing the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5?-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3?) containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt). |
Platform
| Platform | LS454 |
| Instrument Model | 454 GS FLX Titanium |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 0 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Technical Read |
| Read Type | Adapter |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 5 |
