Title | G8VDHLE01__ACGTAGGA__rbcL |
Design Description | Each root tip was washed in 70% ethanol using TissueLyser II (QIAGEN) and 1-mm zirconium balls. The washed root tips were cooled down at -20 degrees C and were pulverized using TissueLyser II and 4-mm zirconium balls. Metagenomic DNA was extracted from each root tip by CTAB method. |
Organism | rhizosphere metagenome |
Name | G8VDHLE01__ACGTAGGA__rbcL |
Strategy | AMPLICON |
Source | METAGENOMIC |
Selection | PCR |
Layout | SINGLE |
Construction Protocol |
Platform | LS454 |
Instrument Model | 454 GS Junior |
PipeSection | |
---|---|
Step Index | 1 |
Prev Step Index | NIL |
Program | |
Version |
Number of Reads per Spots | 0 |
Spot Length | 0 |
Read Index 0 | |
---|---|
Read Label | |
Read Class | Technical Read |
Read Type | Adapter |
Base Coord | 0 |
Read Index 1 | |
Read Label | |
Read Class | Technical Read |
Read Type | BarCode |
Base Coord | 0 |
Read Index 2 | |
Read Label | |
Read Class | Technical Read |
Read Type | Primer |
Base Coord | 0 |
Read Index 3 | |
Read Label | |
Read Class | Application Read |
Read Type | Forward |
Base Coord | 33 |