FASTQExperiment Detail
| Title | Draft genome sequence of Bacillus subtilis Miyagi-4, an efficient starter strain for natto (fermented soybean) production |
| Design Description | |
| Organism | Bacillus subtilis Miyagi-4 |
Library Description
| Name | |
| Strategy | WGS |
| Source | GENOMIC |
| Selection | RANDOM |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 500 |
| Nominal Sdev | 100.0 |
| Construction Protocol | Genomic DNA of B. subtilis Miyagi-4 was isolated from Luria Broth culture according to a routine biochemical isolation procedure. RNase-treated genomic DNA was used for construction of a paired-end library with a Paired End Sample Prep kit (Illumina, San Diego, CA) according to the manufacturer?s instructions. The constructed libraries were sequenced (56 cycles, paired-end runs) using an Illumina genome analyzer IIx. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina Genome Analyzer IIx |
Processing
| PipeSection ( ) | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 112 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 56 |
