FASTQExperiment Detail
| Title | cambium region in cryptomeria japonica |
| Design Description | |
| Organism | Cryptomeria japonica |
Library Description
| Name | cambium region during wood formation |
| Strategy | RNA-Seq |
| Source | TRANSCRIPTOMIC |
| Selection | cDNA |
| Layout | SINGLE |
| Construction Protocol | Poly(A)-RNA (mRNA) was enriched using MicroPoly(A)Purist Kit(Ambion), and cDNA library for sequencing was prepared as manufacturer's instructions (cDNA Rapid Library Preparation Method Manual; Roche) using cDNA Synthesis System Kit, Primer "random" and GS FLX Titanium Rapid Library Preparation Kit (Roche). |
Platform
| Platform | LS454 |
| Instrument Model | 454 GS FLX Titanium |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 800 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Technical Read |
| Read Type | Adapter |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Technical Read |
| Read Type | BarCode |
| Base Coord | 0 |
| Read Index 2 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 16 |
