FASTQExperiment Detail
| Title | Genome-wide profiling of PPARg in Adipocytes (3T3-L1 day 8) |
| Design Description | Genome-wide maps of PPARg binding sites in 3T3-L1 adipocytes (on day 8 of differentiation) using ChIP with high-throughput sequencing (ChIP-seq). source:3T3-L1 cells, day 8 of differentiation. |
| Organism | Mus musculus |
Library Description
| Name | HW_PPARg_3T3L1_day8_ChIP-seq |
| Strategy | OTHER |
| Source | GENOMIC |
| Selection | other |
| Layout | SINGLE |
| Construction Protocol | extraction_protocol:ChIP & Generation of characterization of antibodies for human PPARg and human RXRa was performed as descried previously [Wakabayashi K et al. , 2009, Mol Cell Biol 29:3544-3555]. For ChIP using anti-PPARg, RXRa antibodies, 3T3-L1 cells were cross-linked with 1% formaldehyde for ten minutes at room temperature and were prepared for ChIP as described previously. High-throughput sequencing was performed by using the Genome Analyzer System (GA II) (Illumina) as recommended by the manufacturer. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina Genome Analyzer IIx |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | GAPipeline-1.4.0 |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 36 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
