FASTQExperiment Detail
| Title | Mapping of Open Chromatin in Fibroblasts by FAIRE-seq (NIH-3T3) |
| Design Description | Genome-wide maps of open chromatin sites in NIH-3T3 fibroblasts using formaldehyde-assisted isolation of regulatory elements coupled with high-throughput sequencing (FAIRE-seq). source:NIH-3T3 cells. |
| Organism | Mus musculus |
Library Description
| Name | HW_FAIRE_NIH3T3_FAIRE-seq |
| Strategy | OTHER |
| Source | GENOMIC |
| Selection | other |
| Layout | SINGLE |
| Construction Protocol | FAIRE experiments were performed based on a protocol published by Giresi PG, Lieb JD et al [Methods 2009;48:233-239.]. High-throughput sequencing was performed by using the Genome Analyzer System (GA II) (Illumina) as recommended by the manufacturer. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina Genome Analyzer IIx |
Processing
| PipeSection | |
|---|---|
| Step Index | 1 |
| Prev Step Index | NIL |
| Program | GAPipeline-1.4.0 |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 36 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
