DRX000111

Title	2395_3'adaptor
Design Description	At first, 5'/3' adaptors were ligated to the both ends of shortRNAs. The structure is "5'-ACGCTCACAGAATTCAAA-shortRNA(16-51nt)-U-barcode(4nt)-GAATTGTCACGAGGCCAGCGTGC-3'". After RT reaction and PCR amplification, the PCR products were restricted by EcoRI to make cohesive ends on the both ends of shortRNA tags. The structure is "5'-AATTCAAA-shortRNA(16-51nt)-T-barcode(4nt)-G-3'". These shortRNA tags were concatenated to make long fragments, and then the 454 adaptors were ligated to the both ends of concatemers. The number and the direction of concatenated shortRNA tags are various.
Organism	Mus musculus

Platform	LS454
Instrument Model	454 GS FLX

Number of Reads per Spots	0
Spot Length	400

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