FASTQExperiment Detail
| Title | B. subtilis subsp. subtilis genome resequencing September 2008 |
| Design Description | Randomly fragmented by ultrasonic waves. |
| Organism | Bacillus subtilis subsp. subtilis str. 168 |
Library Description
| Name | B.subtilis subtilis 168 Genomic DNA fragments, isolated Aug 2008 |
| Strategy | WGS |
| Source | GENOMIC |
| Selection | RANDOM |
| Layout | PAIRED |
| Orientation | |
| Nominal Length | 153 |
| Nominal Sdev | 29.528 |
| Construction Protocol | The DNA is purified through the ultracentrifugation in presence of cecium-chloride and ethidium-bromide, then fragmented by ultrasonic waves. Fragments around 160bp were isolated by gel electrophoresis. |
Platform
| Platform | ILLUMINA |
| Instrument Model | Illumina Genome Analyzer II |
Processing
| PipeSection | |
|---|---|
| Step Index | |
| Prev Step Index | |
| Program | Solexa primary analysis |
| Version | |
Spot Information
| Number of Reads per Spots | 0 |
| Spot Length | 72 |
Read Spec
| Read Index 0 | |
|---|---|
| Read Label | |
| Read Class | Application Read |
| Read Type | Forward |
| Base Coord | 1 |
| Read Index 1 | |
| Read Label | |
| Read Class | Application Read |
| Read Type | Reverse |
| Base Coord | 37 |
