BioSample Detail : SAMD00002576

Description

Title16S rRNA gene study of soil associated bacteria in paddy fields dressed with standard nitrogen.
Taxonomy ID410658
Organism Namesoil metagenome
DescriptionField metadata., Country: Japan, Latitude: 38-27-39.37' N, Longitude: 141-5-33.33' E, Altitude: 4 m a.s.l., Classification: Gray lowland soil (classification of cultivated soils in Japan, 3rd approximation), Geomorphic Position: Alluvial plain, Slope Characteristics: Flat, Precipitation: Udic soil moisture regime, Water Table Depth: 97 cm, Drainage: Poorly drained, Land Use: Paddy rice field, Parent material: Unconsolidated alluvium, Vegetation: Paddy field after harvest

General Info

OrganizationLaboratory of Environmental Plant Microbiology, Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University

Model

Sample typeGeneric

Attributes

sample_nameDRS000531
sample commentGeneral description. Rice plants (Oryza sativa L. cultivar gNipponbareh) were cultivated in paddy fields dressed with standard N (SN; 30 kg ha-1). Soil was sampled on August 26, 2009 from each fields. We extracted soil DNA and amplified 16S rRNA genes using a primer set 27F, 518R. These PCR primers target the V1 and V3 region.