BioSample Detail : SAMD00000819

Description

Title[Hd-rR x Kaga]F1
Taxonomy ID8090
Organism NameOryzias latipes

General Info

OrganizationArima lab., Department of Informative Genetics, Environment and Genome Research Center, Graduate School of Medicine, Tohoku University

Model

Sample typeGeneric

Attributes

sample_nameDRS001363
sample commentHd-rR females were mated with Kaga males to generate [Hd-rR x Kaga]F1 medaka. Total RNA was extracted using RNeasy mini Kit and RNase-free DNase (Qiagen, CA, USA) from three [Hd-rR x Kaga]F1 adult fishes, and pooled. Four micrograms of total RNA was used for library construction using TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturer’s protocol. Briefly, poly-A containing mRNAs were purified using poly-T oligo-attached magnetic beads. The purified mRNAs were fragmented using divalent cations under elevated temperatures, and then converted to dsDNA by two rounds of cDNA synthesis using reverse transcriptase and DNA polymerase I. After an end repair process, DNA fragments were ligated with adaptor oligos. The ligated products were amplified using 15 cycles of PCR to generate RNA-seq library. Library integrity was verified by Bioanalyzer DNA1000 assay (Agilent technologies). Sequencing was performed in 39bp paired end mode using Genome Analyzer IIx (Illumina).