BioSample Detail : SAMD00000740

Description

TitlePistils of Japanese apricot 'Nanko'
Taxonomy ID102107
Organism NamePrunus mume

General Info

OrganizationLaboratory of Pomology, Faculty of Agriculture, Ehime University

Model

Sample typeGeneric

Attributes

sample_nameDRS012257
KGP_454null
NGP_454null
KP_454null
NP_454null
SP_454null
UP_454null
sample commentPistils were sampled from unopened flowers of Japanese apricot 'Nanko' at the balloon stage of development. Forty pistils were placed on 1% (w/v) agar in each petri dish and incubated at 20°C overnight. After the overnight incubation, three different pollination treatments were conducted for the pistils. Two subsets of the pistils were pollinated by 'Nanko' (self-pollination, SP) or 'Kairyo-Uchida-Ume' (cross-pollination, CP). One third of the pistils were kept unpollinated (UP). The SP and CP pistils were sampled at 3, 6, and 9 hours after pollination. The UP pistils were also sampled at the same times as the pollinated pistils. A total of one hundred pistils equivalent to the number incubated in two and half petri dishes were sampled at each time point after pollination and pooled for the SP, CP, and UP treatments. Thus, three hundred pistils were used for RNA extraction for each treatment.