Project Detail : PRJDB8304

AccessionPRJDB8304
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleEstablishment of a cell-based assay system for measuring the level of Masc-induced dosage compensation in silkworm cultured cells
Project Description
The Masculinizer (Masc) gene encodes a CCCH-tandem zinc finger protein that controls both masculinization and dosage compensation in the silkworm Bombyx mori (Lepidoptera: Bombycidae). We previously measured the masculinizing activity of the lepidopteran Masc proteins and their derivatives using B. mori ovary-derived cell line BmN-4. In this study, we newly established an RNA-seq data-based assay in which the level of B. mori Masc (BmMasc)-induced dosage compensation can be estimated in BmN-4 cells.
Release Date2019-07-16

Grant 1

GrantIdJSPS KAKENHI Grant Number 15H02482
Agency abbreviationJSPS
AgencyJapan Society for the Promotion of Science

Grant 2

GrantIdJSPS KAKENHI Grant Number 17H06431
Agency abbreviationMEXT
AgencyMinistry of Education, Culture, Sports, Science and Technology

Grant 3

GrantIdJSPS KAKENHI Grant Number 16H06279
Agency abbreviationMEXT
AgencyMinistry of Education, Culture, Sports, Science and Technology

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameBombyx mori
Taxonomy ID7091
Strain,breed,cultivarBmN cell