Project Detail : PRJDB8041

Project TypePrimary submission
Project Data TypeGenome Sequencing

General info

Project titleHMPA intake modifies the changes of HFD-associated gut microbial composition
Project Description
Male C57BL/6J mice were divided into two groups of similar average body weight [cellulose-fed (control) group and 3-(4-hydroxy-3-methoxyphenyl)propionic acid (HMPA)-fed group] for 12 weeks in a factorial design. Cecums were collected at after treatments. Cecal DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces by following the manufacturer's instructions. Partial 16S rRNA gene sequences were amplified targeting the hypervariable regions V4 using 515F/806R primers. The amplicons were sequenced using an Illumina MiSeq with MiSeq Reagent 222 kit V3. The libraries were prepared following the Illumina protocol for 8pM: 'Preparing Libraries for Sequencing on the MiSeq'.
Release Date2019-08-19

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMultiisolate




Organism information

Organism namemouse gut metagenome
Taxonomy ID410661

Project Publication

Publication 1

PubMed ID 31075850