Project Detail : PRJDB739

AccessionPRJDB739
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titlediscovery of GH genes using metatranscriptomic approach
Project Description
We performed the metatranscriptomic analysis to explore the novel eukaryotic lignocellulolytic genes from enrichment culture with pretreated-rice straw as carbon source. The eukaryotic mRNA was sequenced on GS FLX Titanium system and assembled. From the result of analyzing of sequence datasets, 147 glycoside hydrolase (GH) genes were indentified and classified into 40 GH families, and almost genes showed less than 70% similarity to known enzymes of eukaryota including thermophilic fungi and alveolata. Furthermore, 14 contigs were predicted to be full-length cDNA. This study shows that metatranscriptomic analysis using next-generation sequencer is possible to not only to access the transcriptome information but also to obtain full-length cDNA sequences.
Release Date2016-02-02

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeEnvironment
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism namecompost metagenome
Taxonomy ID702656