Project Detail : PRJDB7269

AccessionPRJDB7269
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression, detection of mRNA cleavage sites

General info

Project titleGenome-wide analysis of cleavage sites in Lactuca sativa L. cv. Green wave
Project Description
In previous studies, high-throughput approaches for genome-wide profiling of RNA cleavage sites were developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3'gene ; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) using 30 days after germination of Lactuca sativa L. to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.
Release Date2019-09-10
RelevanceAgricultural

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameLactuca sativa
Taxonomy ID4236
Strain,breed,cultivarGreen wave