Project Detail : PRJDB7191

AccessionPRJDB7191
Project TypePrimary submission
Project Data TypeGenome Sequencing

General info

Project titleMicrobiome analysis in mice fed HFD or HFD-Soybean protein isolate
Project Description
Male C57BL/6 mice were randomly assigned to high-fat diet (HFD) or soybean protein isolate-containing HFD (HFD-SPI) for 4 weeks in a factorial design. Fecal droppings were collected at the end of treatment. Fecal DNA was extracted from frozen samples using the FastDNA SPIN Kit for Feces (MP Biomedicals, Santa Ana, CA) per the manufacture's instructions.The V4 region of the 16S rRNA gene was amplified using dual-indexed 515F/806R primers.he amplicons were sequenced using an Illumina MiSeq with a MiSeq Reagent 222 kit V3 (Illumina, USA).The libraries were prepared following the Illumina protocol for 10pM: 'Preparing Libraries for Sequencing on the MiSeq' (part 15039740, Rev. D).
Release Date2019-08-19
RelevanceAgricultural

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMultiisolate
MaterialGenome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism namemouse gut metagenome
Taxonomy ID410661

Project Publication

Publication 1

PubMed ID 30102711