Project Detail : PRJDB7030

AccessionPRJDB7030
Project TypePrimary submission
Project Data TypeGenome Sequencing, Epigenomics

General info

Project titleRNA polymerase I activators count and adjust ribosomal RNA gene copy number
Project Description
Ribosome is the most abundant RNA-protein complex in a cell and many copies of the ribosomal RNA gene (rDNA) have to be maintained. However, arrays of tandemly repeated rDNA genes can lose the copies by intra-repeat recombination. Loss of the rDNA copies of Saccharomyces cerevisiae is counteracted by gene amplification whereby the number of rDNA repeats stabilizes around 150 copies, suggesting the presence of a monitoring mechanism that counts and adjusts the number. Here, we report that in response to rDNA copy loss, the upstream activating factor (UAF) for RNA polymerase I which transcribes the rDNA is released and directly bind to a RNA polymerase II transcribed gene, SIR2 to repress, whose gene products silence rDNA recombination. We show that the amount of UAF determines rDNA copies number that is stably maintained. UAF ensures rDNA production not only by rDNA transcription activation but also by its copy number maintenance.
Release Date2019-01-03

Grant 1

GrantId17H01443
TitleGrant-in-Aid for Scientific Research (A)
Agency abbreviationMEXT
AgencyMinistry of Education, Culture, Sports, Science and Technology (MEXT)

Grant 2

GrantId26640117
TitleGrant-in-Aid for Challenging Exploratory Research
Agency abbreviationMEXT
AgencyMinistry of Education, Culture, Sports, Science and Technology (MEXT)

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialGenome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads, Analysis

Target

Organism information

Organism nameSaccharomyces cerevisiae BY4741
Taxonomy ID1247190
Strain,breed,cultivarYTT66,67,121,157,159,213

Organism Replicon 1

NameSaccharomyces cerevisiae
TypeeChromosome
LocationeNuclearProkaryote
Size14 Mb

General Properties

CellularityUnicellular
ReproductionSexual
Haploid genome size14 Mb
PloidyHaploid