Microbial communities that grow on surfaces of solid materials in the presence of water are known as a biofilm. Our observation suggested that the biofilm community sampled from coastal area that 30km south than Fukushima daiichi nuclear power plant specifically accumulates radiocesium. Even though all other samples, such as macro-algae and macro-benthos, positive for radioactivity contained radiocesium and radiosilver. Therefore, we analyzed the relationship between the microbial consortia within the biofilms and the accumulated elements.
The radioactivity levels of 134Cs and 137Cs were compared against the concentration of these elements given by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The data has been normalized along each element concentration (ICP-MS data) and radio activity (134Cs and 137Cs) direction to merge the element profile dataset and radioactivity data for 134Cs and 137Cs. The microbial consortia identities were generated by high throughput sequencing (HTSeq) of amplicons from V4 region of the small subunit ribosomal RNA gene (ssrDNA) of 16S and 18S ssrDNA obtained from genomic DNA from each biofilm sample. The number of reads of ssrDNA given by the HTSeq was used as relative abundance data to be input to the statistical analysis coupled to element concentration data given by ICP-MS.