Project Detail : PRJDB4469

AccessionPRJDB4469
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleRNA-seq analysis of Drosophila melanogaster embryos infected and uninfected with male-killing Spiroplasma
Project Description
Spiroplasma (Mollicutes) is one of the heritable bacterial endosymbionts of Drosophila species. Several strains like S. poulsonii manipulate host reproduction in a selfish manner. When females of D. melanogaster are infected with natural S. poulsonii strain MSRO (melanogaster sex ratio organism), only male offspring are killed during embryogenesis, and this phenomenon is called male-killing. To understand the molecular mechanism of male-killing, we compared gene expression profiles between MSRO-infected and uninfected embryos of D. melanogaster by using RNA-sequencing (RNA-seq). For embryonic sexing, we employed a transgenic reporter strain Sex-lethal (Sxl)-Pe-EGFP, which expresses GFP only in females. We collected female and male embryos at stage 10-11, when abnormal apoptosis associated with male-killing starts to occur in male progenies. For each sample, we analyzed three biological replicates.
Release Date2016-09-27

Grant 1

GrantIdKAKENHI 22128001
TitleGenetic Bases for the Evolution of Complex Adaptive Traits
Agency abbreviationJSPS
AgencyJapan Society for the Promotion of Science

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameDrosophila melanogaster
Taxonomy ID7227

Project Publication

Publication 1

PubMed ID27650264