Project Detail : PRJDB3895

AccessionPRJDB3895
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleRNA-sequencing, sporangium formation of Actinoplanes missouriensis
Project Description
RNA-sequencing was conducted in the rare actinomycete Actinoplanes missouriensis to analyze gene expression profiles during sporangium formation. The bacterium forms motile zoospore-containing sporangia upon the substrate mycelia under adverse conditions. In this project, A. missouriensis was cultured on a sporangium-forming HAT agar medium for 1, 3, 6 and 40 days. At day 1, A. missouriensis grows as substrate mycelia. At day 3, sporangium formation starts. At day 6, a certain amount of sporangia are formed. And at day 40, much of the sporangia are supposed to be dormant. Total RNAs were extracted at each time point, and RNA-sequencing using Illumina HiSeq2500 sequencer was conducted.
Release Date2016-06-08
RelevanceEvolution
RelevanceEvolution

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveSequence

Target

Organism information

Organism nameActinoplanes missouriensis 431
Taxonomy ID512565