Project Detail : PRJDB3863

AccessionPRJDB3863
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleTranscriptome analysis in MDCK cells on anti-influenza A activity of bakuchiol
Project Description
Bakuchiol is a phenolic isoprenoid compound present in Babchi (Psoralea corylifolia Linn.) seeds. We examined the anti-influenza activity of synthetic bakuchiol using Madin-Darby canine kidney cells. We found that the naturally occurring form, (+)-(S)-bakuchiol, and its enantiomer, (-)-(R)-bakuchiol, inhibited influenza A infection and growth, and reduced the expression of viral mRNAs and proteins in Madin-Darby canine kidney (MDCK) cells. However, the molecular mechanism in anti-influenza activity of bakuchiol remains unclear. Thus, to evaluate its molecular mechanism in host cells, we performed the next generation sequencing analysis of the MDCK transcriptome in cells treated with bakuchiol and influenza A/PR/8/34. These results provide keys to elucidate the molecular mechnism of anti-influenza activity of bakuchiol for us, and these data will contribute to the development of novel approaches to anti-influenza treatments.
Release Date2015-09-19

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameCanis lupus familiaris
Taxonomy ID9615