Project Detail : PRJDB3471

AccessionPRJDB3471
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleHigh-Throughput Sequencing and De Novo Assembly of Red and Green Forms of Perilla frutescens Transcriptome
Project Description
Perilla frutescens var. crispa (Labiatae) has two chemo-varietal forms, i.e. red and green forms of perilla, that differ in the production of anthocyanins. To facilitate molecular biological and biochemical studies in perilla-specialized metabolism we used Illumina RNA-sequencing technology in our comprehensive comparison of the transcriptome map of the leaves of red and green forms of perilla. Sequencing generated over 1.2 billion short reads with an average length of 101 nt. De novo transcriptome assembly yielded 47,788 and 47,840 unigenes in the red and green forms of perilla plants, respectively. Comparison of the assembled unigenes and existing perilla cDNA sequences showed highly reliable alignment. All unigenes were annotated with gene ontology (GO) and Enzyme Commission numbers and entered into the Kyoto Encyclopedia of Genes and Genomes. We identified 68 differentially expressed genes (DEGs) in red and green forms of perilla. GO enrichment analysis of the DEGs showed that genes involved in the anthocyanin metabolic process were enriched. Differential expression analysis revealed that the transcript level of anthocyanin biosynthetic unigenes encoding flavonoid 3’-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase was significantly higher in red perilla, while the transcript level of unigenes encoding limonene synthase was significantly higher in green perilla. Our data serve as a basis for future research on perilla bio-engineering and provide a shortcut for the characterization of new functional genes in P. frutescens.
Release Date2015-06-16

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeOther
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveAnnotation, Expression

Target

Organism information

Organism namePerilla frutescens var. crispa
Taxonomy ID179837
Strain,breed,cultivarvar. crispa f. purpurea (Akajiso, red perilla) and var. crispa f. viridis (green perilla)

Project Publication

Publication 1

PubMed ID26070213