Project Detail : PRJDB3406

AccessionPRJDB3406
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleIdentification of the gene set correlated with Rb-depletion induced spherogenesis
Project Description
We found that Rb inactivation induces sphere-forming activity under 3D culture condition in primary soft tissue sarcoma cells derived from p53 null mice. To elucidate molecular mechanism, we employed RNA-sequence as a discovery platform to identify gene set of Rb-depeltion induced sphere-forming cells. To enrich sphere-forming cells and make the culture condition equivalent to that of parental cells (Primary cells), we relplated sphere-forming cells on normal dishes (Rb shRNA Secondary cells), and obtained total RNA soon after replating. This gene set was highly enriched in genes involved in inflammation, steroid biosynthesis and central carbon metabolisms. Non-target Secondary cells were derived from surviving control cells without sphere-formation under 3D culture condition, and used as control for manipulation of culture condition.
Release Date2019-01-17

Grant 1

GrantId221S0002
TitleGenome Science
Agency abbreviationMEXT
AgencyJapanese Ministry of Education, Culture, Sports, Science and Technology

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameMus musculus
Taxonomy ID10090