Project Detail : PRJDB3195

AccessionPRJDB3195
Project TypePrimary submission
Project Data TypeTranscriptome or Gene Expression

General info

Project titleTranscriptome analysis in Rhizophagus irregularis
Project Description
To identify differentially expressed genes of arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis during AM symbiosis, we performed transcriptome analysis using illumina Hiseq2000. Total RNA was isolated from the fungus in axenic, strigolactone-treated and monoxenic culture. In axenic and strigolactone-treated culture, germinated spores or elongated mycelia were harvested at a day and a week after inoculation, respectively. In monoxenic culture, R. irregularis and Daucus carota or Medicago truncatula were cocultivated, only extraradical mycelia were harvested at 4, 6 and 8 weeks after spore inoculation, respectively.
Release Date2015-10-05

Grant 1

GrantId22128001
TitleGenetic Bases for the Evolution of Complex Adaptive Traits
Agency abbreviationMEXT
AgencyMinistry of Education, Culture, Sports, Science and Technology

Project Type

Sample scope/Material/Capture/Methodology

Sample ScopeMonoisolate
MaterialTranscriptome
CaptureWhole
MethodologySequencing

Objective

ObjectiveRawSequenceReads

Target

Organism information

Organism nameRhizophagus irregularis DAOM 181602
Taxonomy ID747089